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The sample chamber utilizes O-rings to maintain a high vacuum environment. If the O-ring seal is compromised it can cause a vacuum leak in the system. O-rings should be regularly inspected, cleaned, and greased for optimal performance. The exposed surface should be wiped with a dry Kimwipe or lens tissue and re-greased with a thin layer of L-grease (just enough so the surface is shiny) every 10-15 uses. Before every cooldown, or if a leak occurs, inspect the O-rings to ensure they are free from debris and other contaminates.
To protect O-rings, avoid setting them directly on any surface. When not on the sample chamber, the upper vacuum housing should be set on a flattened corner if it is not equipped with protruding bosses that protect the O-rings. Always wear sterile gloves to avoid contaminating surfaces.
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The vacuum control unit provides an inlet for dry nitrogen gas. Although dry nitrogen is optional, it is highly recommended, especially in humid environments. While nitrogen is not used to assist in the cooling of the system, it does help to optimize vacuum levels and keep the system clean and dry.
Nitrogen is used during the following procedures:
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