Cryo-Optic Module

Documentation

Product Name
Cryo-Optic Module
Overview
CryoOptic Upgrade Kit includes vacuum housing, radiation shield, objective heater ring and vacuum-compatible Zeiss objective. Objective options are: 0.90 NA / 100x with 1.0 mm WD & 0.75 NA / x100 with 1.0 mm WD
Documents
Cryo-Optic User Manual v. 1.2
Download

Specifications

Performance Data

Example Measurements

White Light Imaging

A 3D calibration grid was imaged to demonstrate the system resolution. Fig. 1 shows the actual calibration target. Fig. 2 was imaged by our system (Cryostation® s50 - CO), with 14 pixels across the 1.2µm feature, or an effective pixel size less than 0.1µm.

white light

Confocal Imaging

This experiment demonstrates drift free high numerical aperture confocal microscopy of single molecules at 3.5K in a closed-cycle cryostat. The samples are nitrogen vacancy (NV) centers in diamond that are known to be relatively dark single photon sources, which can be imaged only with well corrected microscope objectives with the highest numerical apertures. Fig. 4 shows a 20µm x 20µm view of a Nitrogen Vacancy center. Fig. 5 shows a single dot with a 2µm x 2µm field of view.

NV center

Options

Objective

The Cryo-Optic add-on includes a special vacuum compatible version of a Zeiss Epiplan Objective.

Cryo-Optic objective

Zeiss LD EC Epiplan-Neofluar 100x/0.75 DIC M27

The Zeiss LD EC Epiplan-NeoFluar objectives are classified as the best universal objectives, with best field flattening, and very good correction, with the addition of extra long working distance. Full specifications and transmittance curve on the Zeiss website.

Zeiss LD EC Epiplan-Neofluar 100x/0.90 DIC M27

The Zeiss EC Epiplan-NeoFluar objectives are classified as the best universal objectives, with best field flattening, and very good correction. It is optimized for high contrast with higher NA and resolving power than the standard Epiplan series. Full specifications and transmittance curve on the Zeiss website.

Video Guides

Guide
Description
Alignment, Imaging, and Use of the Cryo-Optic®
This video explains how to align an objective inside the Cryo-Optic®, how to image your sample, and how to use the Cryo-Optic® software interface and secondary back-up heater.
Assembling the Vertical Cryo-Optic®
This video shows how to assemble the Montana Instruments Cryo-Optic® within the 50 mm sample space.
Cryo-Optic®: High NA Imaging
Overview of the Cryo-Optic® technology on the Cryostation®. The Cryo-Optic® integrates a room temperature objective in vacuum into the cryogenic sample space for virtually drift-free high NA imaging at low temperatures. (Note: While this video features our vertical configuration, the Cryo-Optic® technology is also available in horizontal configurations for our larger platform sizes.)